Affinity Purification of Protein Complexes

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Affinity purification of protein complexes.

INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....

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Identifying the topology of protein complexes from affinity purification assays

MOTIVATION Recent advances in high-throughput technologies have made it possible to investigate not only individual protein interactions, but also the association of these proteins in complexes. So far the focus has been on the prediction of complexes as sets of proteins from the experimental results. The modular substructure and the physical interactions within the protein complexes have been ...

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Affinity purification of FLAG - tagged protein complexes : A cautionary tale

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ژورنال

عنوان ژورنال: Cold Spring Harbor Protocols

سال: 2011

ISSN: 1559-6095

DOI: 10.1101/pdb.prot5611