Affinity Purification of Protein Complexes
                    
                        
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                    چکیده
منابع مشابه
Affinity purification of protein complexes.
INTRODUCTION Knowledge of the composition of protein complexes provides key insights into their functions. Immunoaffinity purification provides an effective means for isolating protein complexes and elucidating their composition. Immunoisolation is achieved with antibodies directed either specifically against the proteins of interest or against tags that are coupled to the proteins of interest....
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MOTIVATION Recent advances in high-throughput technologies have made it possible to investigate not only individual protein interactions, but also the association of these proteins in complexes. So far the focus has been on the prediction of complexes as sets of proteins from the experimental results. The modular substructure and the physical interactions within the protein complexes have been ...
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Through two simple changes in the composition of the fixative for freeze-substitution of cryoimmobilized specimens, we are able to ameliorate the reproducible visibility of the plasma membrane in D. melanogaster embryos and larvae. The ultrastructural analysis of membrane-defective phenotypes of embryos fixed by our modified method should allow us a refined view on the plasma membrane and give ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2011
ISSN: 1559-6095
DOI: 10.1101/pdb.prot5611